Equine Herpesvirus Type 1 (EHV-1)

 

Page 2: Exposure of viral cores

 

cores.JPG (17378 bytes)

EHV-1 nucleocapsids penetrated by negative stain, which outlines core particles.

 

 

expcore1.JPG (57725 bytes)

Core particles extruded from disrupted EHV-1 nucleocapsids. The capsid on the left also has lost its pentamers.

 

 

expcore2.JPG (54652 bytes)

EHV-1 cores exposed after disintegration of nucleocapsid shells. The cores consist of coiled strands with a "beaded" appearance.

 

 

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EHV-1 core material, presumably nucleoprotein. The core on the left consists of a coiled strand with a "beaded" appearance. The other micrographs show short fragments of helical fibers remaining when cores are flattened on carbon support films during dehydration.

 

 

These electron micrographs were obtained during collaborative investigations with:

    Gary H. Cohen, Dept. of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pa.

    Roselyn J. Eisenberg, Dept. of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pa.

    William C. Lawrence, Dept. of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pa.

    Carole A. Long, Dept. of Microbiology, Hahnemann Medical College, Philadelphia, Pa.

   Manuel Ponce de Leon, Dept. of Microbiology, School of Dental Medicine, University of Pennsylvania, Philadelphia, Pa.

    Joel B. Sheffield, Dept. of Biology, Temple University, Philadelphia, Pa.

 

References

Vernon, S.K., Lawrence, W.C., and Cohen, G.H. Morphological components of herpesvirus I. Intercapsomeric fibrils and the geometry of the capsid. Intervirology 4:237-248 (1974)

Vernon, S.K., Lawrence, W.C., Cohen, G.H., Durso, M., and Rubin, B.A. Morphological components of herpesvirus II. Preservation of virus during negative staining procedures. Journal of General Virology 31:183-191 (1976)

Vernon, S.K., Lawrence, W.C., Long, C.A., Cohen, G.H., and Rubin, B.A. Herpesvirus vaccine development: Studies of virus morphological components. In New Trends and Developments in Vaccines, ed. by A. Voller and H. Friedman. Chapter 13, pp. 179-210. MTP Press, Ltd., Lancaster (1978).

Cohen, G.H., Ponce de Leon, M., Diggelmann, H., Lawrence, W.C., Vernon, S.K., and Eisenberg, R.J. Structural analysis of the capsid polypeptides of herpes simplex virus types 1 and 2. Journal of Virology 34:521-531 (1980)

Vernon, S.K., Ponce de Leon, M., Cohen, G.H., Eisenberg, R.J., and Rubin, B.A. Morphological components of herpesvirus III. Localization of herpes simplex virus type I nucleocapsid polypeptides by immune electron microscopy. Journal of General Virology 54:39-46 (1981)

Vernon, S.K., Lawrence, W.C., Long, C.A., Rubin, B.A., and Sheffield, J.B. Morphological components of herpesvirus IV. Ultrastructural features of the envelope and tegument. Journal of Ultrastructure Research 81:163-171 (1982)

 

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