Virology Related Sites: Virological Laboratory Techniques Viral Phylogeny Viral Evolution Viral Genome Data Bio-Informatics Related Resources: Viral Images The Big Picture Book of Viruses Journals The Virology Bookshop ATV Home ATV Table of Contents Submit a New Site Frequently Asked Questions David M. Sander, Ph.D. (david.sander@virology.net ) If you are interested in developing a WWW site for your lab or organization, please feel free to contact me for any needed advice and/or assistance.

Virological Laboratory Techniques & Resources BioGuide - PCR - general information about the polymerase chain reaction including primer design, hot-start, etc. BioProtocol, Inc. - This site contains a large collection of high quality protocols for the life science researcher. These protocols include offerings from the virology field of study. The company has a highly reputable editorial board to oversee the quality of its protocol collection as well as an editorial staff committed to procuring and producing the highest quality protocols available on-line. Detailed protocol for gold labeling proteins for electron microscopy. This protocol describes a modified antibody-protein A-gold technique that eliminates the aggregation problem that frequently occurs with the usual procedure. Cell Biology Laboratory Manual by Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College, St. Peter, MN. SPOTTER: a new tool to measure viral plaques. The plaque assay is a classical virological technique, originally developed for bacteriophages by Felix d'Herelle (1917). Dulbecco and Vogt subsequently adapted the assay for mammalian viruses, and it is still widely used for virus isolation and purification, and to determine viral titers. The plaque assay is also frequently used to study in vitro growth properties of mutant viruses. A freeware program,called SPOTTER, operating under Win95 and up, allows convenient and precise analysis of large numbers of plaques. Protocol On-Line - an exhaustive collection of methods and protocols for molecular and cellular biology from academic and comercial laboratories. There is also a Q&A sections that may be helpful for solving lab technique problems. Comprehensive Protocol Collection - This resource attempts to offer a comprehensive collection of molecular biology protocols. The site links to protocols either maintained directly by the Ambros Lab at Dartmouth or at other locations around the world. The HHMI Virtual Lab: This interactive laboratory, developed with Shockwave, demonstrates how an enzyme-linked immunosorbent assay (ELISA) is carried out and some of the experimental problems that may be encountered. A free Shockwave plugin is required. Molecular Biology Protocols - a General Resource from the NOAA of Molecular Methods, Manuals, and some great Links. PCR in situ: New Technologies with Single Cell Resolution for the Detection and Investigation of Viral Latency and Persistance" from the University of Minnesota PCR Jump Station - This site attempts to provide scientists with a comprehensive directory for the polymerase chain reaction (PCR) and related techniques. Labexplorer.com is a new web site written by clinical laboratorians for clinical laboratorians. Designed to be a comprehensive resource for those interested in clinical laboratory practices, the site provides a mechanism of communication between laboratorians, access to experts in a variety of clinical fields, job and career information, and comprehensive links to laboratory sites, diagnostic companies, organizations & interesting topics. Molecular Biology Protocols on the Web - a great collection of protocol links from the publishers of Molecular Biology Today. Taxonomy and Phylogeny of Viruses Don't forget to check the Big Picture Book of Viruses, here at our very own site! Classification and Nomenclature of Viruses - The Index Virum presents lists of virus taxa that reflect the currently approved classification of the International Committee on Taxonomy of Viruses (ICTV). The ICTV Web Page is up and running at the National Center for Biotechnology. From Wisconsin: Sequence Relationships and Taxonomy Baltimore Classification of Viruses In the Beginning... The Tree of Life Home Page Virus Evolution Noble Foundation Virus Evolution Page - This site is dedicated to the study of virus evolution. It includes a list of current references, web links, meeting lists and abstracts from the 1999 Virus Evolution Workshop. Genomic Sequence Data of Viruses Don't miss my own articles on WWW resources for genomic sequence data and analysis. From the Animal Virus Information System Site, several utilities in this area are available. These include FASTA for viral sequences - This utility allows the user to carry out the FASTA analysis with a variety of protein sequence libraries. These libraries include, the standard PIR databases, NRL-3D database and several libraries created for individual virus families. The results also lists the virus families to which the sequences belong. Also Available: BLAST for viral sequences - This utility allows to carry out the BLAST analysis with a variety of protein sequence libraries. MATCH a peptide among viral sequences - This utility allows to match a peptide with a variety of protein sequence libraries. SCAN a peptide among viral sequences - This utility allows to scan a peptide with a variety of protein sequence libraries. Geminivirus Sequences Herpesvirus sequence database at University College London : The site provides a clikable list of all completely sequence herpesvirus genomes. The user can also fetch herpesvirus protein sequences in Fasta format using keywords or GenBank GI code. There are also links to various sites for analysis of proteins. Los Alamos HPV Database Los Alamos HIV Database Picornavirus Sequence Database Ann Palmenberg's Picornavirus Sequence Alignments General Sequence Data, Analysis and Searches: ANU Molecular biology BCM Search Launcher, Human Genome Center Codon Usage Tools EMBL sequence data EMBL Data Library directly from Heidelberg EC enzyme database Emerging Infectious Diseases Resources for Protein, NA, & 2D-PAGE Genome Databases Genome Workstation GeneCards: 6000 gene database Genbank Submission (BankIt!) Human 2-D PAGE Databases Human Genome Center: All BCM Searches Internet Drug Index Johns Hopkins Bio-Informatics Web Server Medline Molecular Genetics Metabolic Database Newsgroups National Center for Biotechnology Information NCBI : BLASTsearch through the National Library of Medicine NCBI Entrez browser NCBI dbEST Expressed Sequence Tags NCBI Genbank Database NCBI Molecular Modeling Database NCBI Nucleotide Database NCBI Protein Database Pedro's Biomolecular Research Tools Primer Selection The Protein Data Bank provides a searchable database for information on protein, DNA, RNA, virus and carbohydrate structures. Thirteen possible fields, including author and compound, allow for detailed searches. FTP and gopher sites are also available, and a listserv FAQ explains the basics. Newsletters, image files, links to related databases and WWW sites are also provided. Proteome Analysis: DCHGR Search for related peptide sequences REBASE (Restriction Enzyme Database) Rnadraw Homepage Swiss-Model: Automated Protein Modelling Server Table of Standard Genetic Code Transcription Factor Database Tree of Life Vectors, (Japan) Vectors, Johns Hopkins Vector DB WWW DNA & Protein Analysis Toolkit Bio-Informatics Bioinformatics Help BBS -- A free service to encourage information sharing on the subject of bioinformatics and related topics within the biotech community. Features Q & A on databases, search strategies, and analysis tools. Home | Table of Contents | FAQ Submit a New Site

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