Research Technician
The University of Pittsburgh
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Post Date: Mar 23, 2017
Job Type: Full time
Degree Requirement: Bachelors
Years Experience: 2+
Location: US - Pennsylvania - Pittsburgh
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Chikungunya Virus Research Technician:
The Department of Pediatrics at the University of Pittsburgh is seeking a highly-qualified, motivated, and detail-oriented person for the position of Research Technician (III) in the Dermody Lab. The successful candidate will join a dynamic, multidisciplinary team with diverse backgrounds and work in a well-equipped, interactive research environment at the Rangos Research Center at Children's Hospital of Pittsburgh in Lawrenceville. The position calls for creativity, multitasking, problem solving, and the desire and ability to conduct independent studies in a team setting. Our research focuses on replication and pathogenesis of chikungunya virus (CHIKV) and the position will include supervised and independent research at BSL-2 and BSL-3 conditions consisting of 1) classic virological assays of CHIKV; 2) molecular biology-based assays to uncover mechanisms of CHIKV replication; and 3) pathogenesis studies using mouse models of disease. The technician will assist in mammalian specimen breeding, husbandry, and animal experiments. The successful applicant will provide research support to other members of the team and will manage an independent research project, given sufficient training, familiarity, and proficiency. The technician will be expected to perform complex lab testing, help develop research methods and procedures, and organize data and laboratory samples.
Research duties include:
•        Assisting in performing of virologic assays in BSL-2 and -3 laboratories. Helping to develop basic design, execution, and interpretation of a variety of laboratory experiments including but not limited to cell culture, nucleic acid extraction, molecular cloning, sequence analysis, confocal microscopy, western blots, PCR, and viral titer determination.
•        Maintaining and expanding continuous mammalian and insect cell lines.
•        Generating and maintaining viral stocks.
•        Maintaining records of tests performed and data collected, and compiling and analyzing data.
•        Reading and familiarizing with relevant literature.
•        Participating and presenting at regular lab meetings, journal clubs, and working group meetings.
•        Work with animals includes:
•        Maintenance of subset of animal colony.
•        Assisting with and independently perform animal experiments in a BSL-3 laboratory:
o        Perform subcutaneous infections
o        Daily supervision of animals during experiments
o        Tissue harvesting and processing
o        Organizing and maintaining sample inventories
•        Cage management
•        Must adhere to established BSL3 practices and follow specific safety SOPs without deviation.
General lab duties include:
•        Ordering and maintaining lab inventory of supplies, reagents, and animals.
•        Preparing and disposing of lab reagents and solutions.
•        Operating, maintaining, and evaluating performance of lab equipment.
•        Coordinating lab equipment repairs with manufacturers.
•        Maintaining a safe and secure work environment.
•        Additional duties as the need arises.

•        Aptitude for detail-oriented mindset, efficient time management, and analytical, problem-solving skills. Interpersonal skills to effectively interact with supervisors, other lab members, and vendors. An open and collaborative personality is highly preferred.
•        Experience working with small animal models is required and experience with infection of animals is preferred.
•        Experience working in BSL3 conditions would be considered highly advantageous.
•        Genuine interest in laboratory research and a desire to learn and contribute to a group effort.
•        Ability to learn new techniques and self-sufficient to identify deviation from processes and promptly report issues that may impact safety, security, or technical reliability of data.
•        Proficiency and clarity in oral and written communication, including maintenance of an accurate lab notebook, contributing to scientific analysis of projects, and manuscript preparation.
•        Ability to perform or learn data entry and basic data analysis using relevant mathematical and statistical analysis and computerized data processing (Adobe Photoshop and Illustrator, GraphPad Prism, and Image J). Ability to know or learn basic software programs (Microsoft Word, Excel, and PowerPoint, Adobe Acrobat, and EndNote).
This position is located at Children's Hospital of Pittsburgh of UPMC in Lawrenceville.

A bachelor's degree in biology, chemistry, or other basic science is required. A background in clinical microbiology or bacteriology and/or formal Medical Technologist certification (with a focus on microbiology) is highly desirable but not required. A minimum of two to three years of research experience required.
•        Occasional work after business hours or on weekends according to project demands.
•        Chikungunya virus is categorized as a biosafety level-3 laboratory (BSL-3) pathogen. Applicants should be comfortable with wearing an N-95 mask or powered air purifying respirator (PAPR) and other personal protective equipment and adhering to established BSL3 protocols.
•        This position is located at Children's Hospital of Pittsburgh of UPMC in Lawrenceville.
•        TB test and PA Child Abuse History Clearance, PA State Police Criminal Record Check and FBI Criminal Record Check will be required as a condition of employment.
•        EEO/AA/M/F/Vets/Disabled.

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