NIH-funded postdoctoral positions to study virus assembly, vaccine/antiviral development, or bacterial appendages and secretion systems are available in the Wright lab (http://electronmicroscopy.emory.edu). Major research efforts in the lab are focused on 3D structure/function relationships between host cells and viruses or bacteria. Areas of particular interest include cryo-electron tomography of bacteria, bacteria – bacteriophage interactions, paramyxoviruses, pneumoviruses, and HIV-1. Technology development interests are in the areas of correlative microscopy and phase plate cryo-microscopy.
Publications applicable to the projects associated with the position(s):
1. C. M. Hampton, J. D. Strauss, Z. Ke, R. S. Dillard, J. E. Hammonds, E. Alonas, T. M. Desai, M. Marin, R. E. Storms, F. Leon, G. B. Melikyan, P. J. Santangelo, P. W. Spearman, and E. R. Wright. “Correlated Fluorescence Microscopy And Cryo-Electron Tomography Of Virus-Infected And Transfected Mammalian Cells.” Nature Protocols. 2017: 12 (1): 150-167.
2. C. C. Stobart, C. A. Rostad, Z. Ke, R. S. Dillard, H. Yi, A. L. Hotard, J. Meng, R. J. Pickles, K. Sakamoto, S. Lee, M. G. Currier, S. M. Moin, B. S. Graham, M. S. Boukhvalova, B. E. Gilbert, J. C. G. Blanco, P. A. Piedra, E. R. Wright, and M. L. Moore. "A Live-Attenuated RSV Vaccine With Increased Incorporation Of Pre-Fusion F Exhibits Enhanced Thermal Stability And Immunogenicity." Nature Communications. 2016: 7: 13916.
3. R. C. Guerrero-Ferreira, P. H. Viollier, B. Ely, J. S. Poindexter, M. Georgieva, G. J. Jensen, and E. R. Wright. “Alternative Mechanism For Bacteriophage Adsorption To The Motile Bacterium Caulobacter crescentus.” Proc Natl Acad Sci U.S.A. 2011: 108(24): 9963-9968.
The wet laboratory is within the Division of Pediatric Infectious Diseases and is fully equipped for all aspects of microbiology, molecular biology, molecular virology, and protein biochemistry. The Wright lab is equipped with a JEOL JEM-2200FS 200 kV FEG TEM with an in-column energy filter, phase plate system, Direct Electron DE-20 direct electron detector, and Gatan 4kx4k CCD camera. This instrument will also serve as a feeder microscope to FSU and other cryo-EM consortia with Titan Krios cryo-microscopes. For cryo-CLEM development and experiments, there is a Leica cryo-CLEM system and access to multiple live-cell imaging platforms. The laboratory also has an FEI Vitrobot, a Gatan CP3 and a manual grid plunge freezer; and a Leica cryo-ultramicrotome. The group has access to all the resources available in the Emory University EM Core Facility, to include: a JEOL JEM-1400 120 kV TEM with Gatan 2kx2k CCD camera; a Bal-Tec high-pressure freezer; and Leica freeze substitution system.