Post-Doctoral Fellow - Viral RNA capping
Brian Geiss
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Post Date: Feb 16, 2018
Job Type: Full time
Degree Requirement: Ph.D.
Years Experience: Entry Level
Start Date: 4/1/2018
Salary: $48,000
Location: US - Colorado - Fort Collins
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We are looking for an enthusiastic and motivated post-doctoral fellow to contribute to a newly funded NIH R01 project focused on defining the role of uncapped viral RNAs in the flavivirus replication cycle. Flaviviruses, including dengue, West Nile, and Zika viruses, produce and package a significant amount of uncapped RNAs during their replication that may act as substrates for the RNA decay pathway. Intriguingly, a pseudoknot structure in the flavivirus 3’ UTR inactivates 5’ exonucleases and alters the function of the RNA quality control system in cells, protecting the viral RNAs from degradation and enhancing virus pathogenicity. It is currently unclear how RNA structures within the viral 5’ untranslated region contribute to RNA capping efficiency and how capped and uncapped viral RNA species interact with the RNA decay pathways in cells. Defining which viral RNA species inhibit RNA decay components and when inhibition occurs during infection will provide new fundamental knowledge of flavivirus genome replication and how RNA capping efficiency influences viral pathogenesis.

We are looking for a post-doctoral fellow with significant experience in RNA biology (experimental design, RNA transcription, and testing for capping of RNA transcripts), cell biology (knockdown of cellular gene expression and validation) and BSL-2 cell culture and virology. Experience with protein biochemistry (expression, purification, and analysis) and/or mass spectroscopy of nucleic acids is preferred.

Key goals for this position will be to 1) explore how the structure of the 5’ UTR influences RNA capping efficiency, 2) define the efficiency of viral RNA capping in vivo, 3) determine how ablation of cellular RNA decay pathway components affect genome replication and RNA species distribution, and 4) assess the effect of packaged, uncapped viral RNAs on infection efficiency.

For full position announcement and to apply go to:

CSU is an EO/EA/AA employer and conducts background checks on all final candidates.

Contact Details
Name: Colorado State University
Division: Geiss Lab
Department: Microbiology, Immunology, and Pathology
Address: 1682 Campus Delivery
City: Fort Collins
State/Province: Colorado
Zipcode: CO
Country: United States
Ph: (970) 491-6330